GCSAT :: Volume #4

#321: Saturated type experiment


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Gui Sheng, yl City Institute of Agricultural Sciences. 桂省,yl市农科院。 The ultra-clean vaccinates indoor work area, two researchers who wear the whole body exposure suits, not to distinguish the men and women is making one to compare with this high-end atmospheric laboratory as if has a little not on the work of scale. 超净接种室内的工作区,两名身穿全身防护服、不辨男女的研究员正在做着一项跟这个高端大气的研究室相比似乎有那么一点不上档次的工作。 They in exhausted rough rice. 他们在剥稻谷。 In front of two people is suspending full one bottle already the rough rice seed that undergoes the careful choice, these seeds each one full gigantic, is golden yellow, superficial has not gotten moldy and spoil, belongs superiorly chooses the superior rough rice. 两人面前摆着满满一瓶已经经过精心挑选的稻谷种子,这些种子个个都饱满硕大,色泽金黄,表面没有一丝霉变,属于优中选优的稻谷。 But two people must do, glances at several hundred rough rice to take off the hull this completely manually. 而两人要做的,就是把这粗看过去有数百颗的稻谷全部手工脱去谷壳。 This is a tedious and arid work, but their looks are very dedicated, besides chatted several occasionally mutually, does not have any unnecessary movement. 这是一项繁琐且枯燥的工作,但他们的眼神却无比专注,除了偶尔相互聊几句之外,没有任何多余的动作。 Has peeled to the eye turns sour, a talent says: 一直剥到眼睛发酸,其中一人才开口说道: senior brother, I am not good, this gadget too fee/spent eye. How many did you peel?” “师兄,我不行了,这玩意儿太费眼睛了。你剥了多少颗了?” The Senior Brother in this female accent looked at a triangle bottle that one side places the pushing crowded jostlingly, says: 这个女声口中的师兄看了一眼放在一旁挤挤挨挨的三角瓶,开口说道: 169. My this quick Group 17, your?” “169颗。我这已经快17组了,你那呢?” My this Group 12. Yeah, my hand too fresh, the exhausted millet peels not quickly. senior brother, do we make the induction today also with enough time?” Remembers the website “我这12组。哎,我手还是太生了,剥个谷子都剥不快。师兄,咱们今天做诱导还来得及吗?”记住网址 The induction in female student mouth, refers to the paddy rice callus induction, this conducts the first step of gene edition experiment, only then after completing the induction, can with change over to the agrobacterium tumefaciens that the gene has edited the carrier to invade the callus, thus changes the paddy rice gene the character, the goal of achieving the gene edition. 女生口中的诱导,指的是水稻愈伤组织诱导,这是进行基因编辑实验的第一步,只有完成诱导后,才能用已经转入了基因编辑载体的农杆菌去侵染愈伤组织,从而改变水稻基因的性状,达成基因编辑的目的。 After the callus completes the induction, they must continue to make the agrobacterium tumefaciens activation and expanded training, to invade and train and screen training, emerge to take root...... 而在愈伤组织完成诱导之后,他们还要继续去做农杆菌活化、扩大培养、侵染和共培养、筛选培养、出苗生根...... These all steps complete, observes one to two weeks later, after the paddy rice of gene edition can be transplanted to be buried, after waiting to be mature, observes. 这些所有的步骤做完,观察一到两周之后,一株经过基因编辑的水稻才能被移栽入土,等成熟之后再进行观察。 This is a long process, although without other physics or the chemistries is so precise to the control of atom, but if any step gave rise to the problem, brought will be the failure of entire experiment. 这是一个漫长的过程,虽然没有其他物理或者化学那样精确到原子的控制,但如果其中任何一个步骤发生了问题,带来的都将是整个实验的失败。 The male students put down the rough rice in hand, flung the wrist/skill to move the neck gently, later opened the mouth to reply: 男生放下手里的稻谷,轻轻甩了甩手腕又活动了脖子,随后才开口回答道: „The present is 3 : 00 pm, we altogether must make Group 50 to induce, by our two was definitely without enough time, little Liu they did not come after a while, the words that tonight works overtime, should be able to complete reluctantly.” “现在是下午 3 点,咱们总共要做50组诱导,靠我们两个是肯定来不及了,不过一会儿小刘他们就过来了,今天晚上加个班的话,应该勉强能做完。” Was late completed, I led you to go to the Lizhiyuan of Boss king to steal the litchi chinensis to eat ---- This time you study intelligently, do not catch a tree to weed out, he tree in the roadside, full, that you who nearby tree ties steal bare, you said that you are scolded?” “晚点儿做完了,我带你去王老板的荔枝园偷荔枝吃----这次你学聪明点,别逮着一棵树薅了,那他么树就在路边,旁边的树都结的满满的,就你偷的那棵光秃秃的,你说你挨不挨骂?” In the look of female student disclosed that a grievance, she mumbled ambiguously several, then said: 女生的眼神中透露出一丝委屈,她含含糊糊地嘟哝了几句,然后说道: Their litchi chinenses planted to come out are not let the person ate...... not test the variety, ate to eat......” “那他们的荔枝种出来不就是让人吃的嘛......又不是实验品种了,吃点就吃点呗......” „Is key point you steals the litchi chinensis? What meaning did yourself recall Boss king well at that time that complexion are? Others pick the litchi chinensis to know that this picks a point that to pick you to select, you? I watch you, the fruit of entire tree made you pick!” “重点是你偷荔枝吗?你自己好好回忆回忆王老板当时那脸色是啥意思?别人去摘荔枝都知道这摘一点那摘你点,你呢?要不是我看住你,整棵树的果子都让你摘完了!” „...... Knew, is dislikes me to be stupid.” “......知道了,就是嫌我笨呗。” Doesn't dislike you to be stupid? Ok, do not put in great inconvenience, others criticize your two to joke with you, in our institute isn't that litchi chinensis which year the person on one's own side first eats half? Toward do not go on the line at heart.” “可不是嫌你笨吗?好了,别委屈了,人家批评你两句就是跟你闹着玩的,咱们院里那荔枝哪年不是自己人先吃一半?别往心里去就行。” The female students nod, replied: 女生点点头,回答道: I toward have not gone at heart, I felt oneself very silly......” “我没往心里去,我就是觉得自己挺傻的......” Is saying, she while lifts the hand to rub the eye subconsciously, was stared by opposite male student, frightens to hurry the hand to put. 一边说着,她一边下意识地抬起手想要揉眼睛,被对面的男生一瞪,吓得赶紧把手放了下来。 The stop a moment later, she is taking to sigh aseptically, then opens the mouth to ask: 停顿片刻之后,她在无菌服里叹了口气,然后开口问道: senior brother, you said that our experiments can succeed? Is that ac-bemax editor credible?” “师兄,你说咱们这次实验能成功吗?那个ac-bemax编辑器到底靠不靠谱啊?” Hears the issue of female student, the male student was silent was thinking deeply about the moment, then replied: 听到女生的问题,男生沉默着思索了片刻,然后回答道: I have read the ac-bemax documents, “我看过ac-bemax的文档, This editor is not that you think has the new experiment editor that every year, it is one complete, after experimental data confirmation and correction, the stable very high editor, from the performance and usability, this model of editor are nothing issues. ” But the issue is, although this model of editor fused in the world the characteristics of many editor, but has distinguished with all editors, therefore studies this model of editor from the beginning, actually needs to spend a lot of time.” 这个编辑器不是你想的那种每年都有的新出现的实验型编辑器,它是一个完整的、经过实验数据验证和调校的、稳定性很高的编辑器,从性能和实用性上来说,这款编辑器是没有任何问题的。”“但是问题是,这款编辑器虽然融合了世面上很多编辑器的特点,但是又跟所有的编辑器都有所区别,所以从头去学习和熟悉这款编辑器,其实是需要花费很多时间的。” Good, now the contradictory place came, we do not have so many time to study. Because of according to the above arrangement, realizes the topic of production increase for dealing with that side ugly country the grain campaign through the krn2 edition that this time we study, must make the achievement with the quickest time.” “好,现在矛盾的地方来了,我们没有那么多的时间去学习。因为按照上面的安排,这次我们研究的通过krn2编辑实现增产的课题是用于应对丑国那边的粮食战役的,必须用最快的时间来做出成果。” Therefore the difficulty is also very obvious, how we must balance the good speed and accuracy, how to guarantee, when uses this model of editor, disposable obtains the success.” “所以困难也很明显,我们要怎么样平衡好速度和准确性,怎么保证在使用这款编辑器的时候,一次性就取得成功。” This is the biggest problem that we face now.” “这才是我们现在面临的最大困难啊。” The opposite is listening to the female student nods looking pensive, then continues to ask: 对面听着的女生若有所思地点了点头,然后继续问道: What therefore the solution is? Can't we bet the luck?” “所以解决方法是什么?总不能我们就赌运气吧?” The male students smile gently, replied: 男生轻轻笑了笑,回答道: You let alone, but also really bets the luck. However, we bet the luck the way to be a little special ---- Roams about Earth to look? Inside has a concept to be called the saturated type rescue.” “你别说,还真是赌运气。不过,我们赌运气的方式有点特别----流浪地球看过吗?里面有个概念叫做饱和式救援。” Now we do is, saturated type experiment.” “我们现在做的就是,饱和式实验。” According to the message that I received, the nation all has the condition to make the agricultural center and Institute of Agricultural Sciences of gene edition experiment to start to contact this model of editor, we were earliest one batch, start one batch of vaccination early.” “根据我收到的消息,全国所有有条件做基因编辑实验的农科所、农科院都已经开始接触这款编辑器了,我们算是最早使用的一批、也是最早启动接种的一批。” If our experiment failure, that across the country also about hundred times opportunities.” “如果我们的实验失败,那全国范围内还有近百次的机会。” In these opportunities, even if only succeeded one time, even if only supported one, then also means our experiment successes.” “这些机会里,哪怕只成功了一次,哪怕只养活了一株,那么也意味着我们这次的实验成功了。” Although bets the luck, but we are making best effort, removes these uncertainty.” “虽然都是赌运气,但我们是在尽最大的努力,去排除掉那些不确定性。” With vulgar chicken soup, probably is, diligently, is luckier?” “用一句庸俗的鸡汤来说,大概就是,越努力,越幸运?” Hehehe...... seems like such truth. senior brother, if our time experiments successfully, you estimated the paddy rice that this new variety how many can increase production?” “嘿嘿嘿......好像是这么个道理。师兄,如果我们这次实验成功的话,你预计这个新品种的水稻能增产多少啊?” Theoretically about 20 ---- This mainly looked ties the situation in Guangzhou finally. When the time comes you look, go to the field to walk frequently, do not let the bird give our experimental field to spoil.” “理论上在20左右吧----这主要还是看最后结穗的情况了。到时候你看着点,经常去田里走走,别让鸟给我们的实验田糟蹋了。” Knows that knows. senior brother, do you go with me when the time comes together?” “知道啦知道啦。师兄,你到时候跟我一起去呗?” „Can I not go with you together? Let your girl alone the patrolling field, my backbone must be poked.” “我能不跟你一起去吗?让你一个女孩子单独去巡田,我脊梁骨都要被人戳断。” Hears the words of male student, in the eyes of female student exudes a sweet intent, but also is faint some suspicions and disappointments. 听到男生的话,女生的眼里泛起一丝甜意,但又隐隐有些怀疑和失望。 However, this disappointment was continued the arid work to dilute quickly, they sit facing each other in the laboratory, when has bustled about to around 7 : 00 pm, completed the depilation work of complete seed. 不过,这种失望很快被继续进行的枯燥工作冲淡,他们在实验室里相对而坐,一直忙碌到晚上7点多时,才完成了全部种子的去壳工作。 But after conducting this, they did not have the opportunity to go out to rest. 而进行到这一步之后,他们也没有机会在出去休息了。 If not complete the explant disinfection and vaccination immediately, these seeds expose the time in air are longer, on testing stable the effect is also bigger. 如果不立刻完成外植体消毒和接种,这些种子暴露在空气中的时间越久,对实验稳定性的影响也就越大。 But the following work load is really big, by them, feared that is an evening cannot complete. 但接下来的工作量实在是太大,靠他们两个人,怕是一晚上都做不完。 ---- Is good after they operated independently for a half hour, agrees the good reinforcements to rush in abundance, in the aseptic vaccination room immediately becomes lively, chat was operating on the colleagues of getting to know each other well , the time arrived at 12 points. ----好在他们独立操作了半个小时之后,约定好的援军纷纷赶到,无菌接种室里立刻变得热闹起来,原本就相熟的同事们说说笑笑地在操作着,不知不觉间,时间就走到了12点。 After completing the seal of last culture dish, the people go out of the sterile room together, when they take off the exposure suit arrives in the hall the time, sees on the floor of hall corner, had displayed the thing chock full. 在完成最后一个培养皿的封口后,众人一起走出无菌室,等他们脱下防护服走到大厅里的时候,才看到在大厅一角的地板上,已经满满当当地摆上了东西。 But before that criticizes female student to steal Boss king who the thing will not steal, at this moment takes a seat sits by that bunch of things, the mouth is holding in the mouth the smoke, a drowsy appearance. 而那个之前批评过女生“偷东西都不会偷的”王老板,此刻就席地坐在那一堆东西旁边,嘴里叼着烟,一副昏昏欲睡的样子。 Sees the people to come out, he shook the head to stand up, arrives in front of the female student saying: 看到众人出来,他晃了晃脑袋站起身,走到女生面前说道: Come, doesn't like eating the litchi chinensis? This time makes you eat to the full!” “来,不是爱吃荔枝吗?这次让你吃个饱!”
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